Innovative UPLC-MS/MS method for quantification of 1α-hydroxycorticosterone in plasma, as biomarker for acute stress in sharks


StressChron Framework:

Project code111
Research areaContributing to a more sustainable and animal welfare minded food
Research topicImproving our understanding of the impact of anthropogenic activities on
the health status of vertebrates in the wild
SpeciesDogfish (Squalus acanthias)



The research goal is the development of an ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method for quantification of 1α-hydroxycorticosterone as active glucocorticoid in the plasma of sharks in an EN ISO/IEC 17025 regulated environment and analytical validation according the requirements of the Commission Decision No. 2002/657/EC. Biological validation is done by assessing 1α-hydroxycorticosterone using UPLC-MS/MS in samples from unstressed and stressed dogfish (Squalus acanthias).



Keywords: wildlife, fish, shark, stress, 1α-hydroxycorticosterone, plasma

Funding: internal

Status: on-going


Ghent University (Aerts J)

Flanders Research Institute for Agriculture, Fisheries and Food (Aerts J)

Universidad de Cadiz, Spain (De La Rocha IRJ, Mancera JM)



Ruiz-Jarabo I, Barragan-Mendez C, Jerez-Cepa I, Fernandez-Castro M, Sobrino I, Mancera JM*, Aerts J* (2019) Plasma 1α-hydroxycorticosterone as biomarker for acute stress in catsharks (Scyliorhinus canicula) Front Physiol 10:1217 (Q1 - IF=4.134)
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