Optimization of fish diets by inclusion of lupine meal and its potential effect on the chronic stress level in European sea bass (Dicentrarchus labrax)


StressChron Framework:

Project code087
Research areaUnravelling the impact of chronic stress across vertebrates
Research topicFeed as stressor focusing on invertebrates (e.g. Artemia), mycotoxins
and fish feed constituents
SpeciesEuropean sea bass (Dicentrarchus labrax)



The research goal is to assess the effect of the inclusion of different levels of lupine meal in the normally animal based diet of European sea bass (Dicentrarchus labrax) on the hypothalamic-pituitary-interrenal (HPI) axis activity by quantification of scale cortisol using ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) as biomarker for chronic stress in fish.



Keywords: aquaculture, fish, stress, diet, optimization, cortisol, scale

Funding: internal

Status: on-going


Ghent University, Belgium (Aerts J)

Flanders Research Institute for Agriculture, Fisheries and Food, Belgium (Aerts J)

Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Germany (Hörterer C, Slater MJ, Zeytin S)



Zeytin S, Aerts J, Hörterer C, Slater MJ. (2018) Chronic stress effect of high levels of lupin meal inclusion in diets revealed by scale cortisol in European sea bass (Dicentrarchus labrax). European Aquaculture Society, Dubrovnic, Croatia, October 20th 2018, abstract.

Aerts J. Affecting the stress response in fish: The increasing usage of alternative fish meal sources. Int. Aquafeed January 2018, article.